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KMID : 0861020090240040137
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Korea Journal of Herbology
2009 Volume.24 No. 4 p.137 ~ p.142
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Antioxidant Activity of Isolated Compounds from the Shoot of Aralia elata Seem
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Lee Gi-Ho
Jung Ji-Wook
Ahn Eun-Mi
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Abstract
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Objectives: This study was performed to investigate the antioxidant activities of isolated compounds from the shoot of Aralia elata.
Methods: The methanol extract from the shoot of Aralia elata was fractionated into ethyl acetate, n-BuOH and H2O layers through solvent fractionation. Repeated silica gel, ODS column chromatography of n-BuOH layer afforded four flavonol glycosides. Their antioxidant activity was determined by measuring free radical scavenging activity by DPPH, ABTS and superoxide dismutase (SOD) like activity assay.
Results: They were identified as quercetin 3,7-di-O-α-rhamnopyranoside (1), quercetin 3-O-β- D-galactoside-7-O-α-L-rhamnoside (2), kaempferol 3-O-β-glucosyl(1→2)-α-rhamnoside-7-O-α- rhamnoside (3) and quercetin 3-O-β-glucosyl(1→2)-α-rhamnoside-7-O-α-rhamnoside (4) on the basis of spectroscopic data. The result showed that 1 is the most active compound in the DPPH and ABTS radical scavenging test.
Conclusions: Isolated Compounds from the shoot of Aralia elata showed anti-oxidative effect.
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KEYWORD
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Aralia elata, antioxidant activity, flavonol glycosides
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